Lack of a clear tumor boundary hampers the surgeon’s ability to accurately detect and resect infiltrating tumor tissue. Higher harmonic generation enables label-free imaging of excised brain tissue, revealing histological hallmarks within seconds. The combination of real-time imaging and image analysis shows great potential for interoperative assessment of brain tissue during tumor surgery.
Intraoperative histology for brain tumors
In diseases such as interstitial lung diseases, immediate feedback on leukocyte ratios has potential to speed-up the diagnostic process and to reduce costs, workload and inter-observer variations. Studies have shown promising leukocyte identification performance on blood fractions, using third harmonic generation (THG) and multiphoton excited autofluorescence (MPEF) microscopy.
HHG imaging for Interstitial Lung Disease
Rapid and reliable histology assessment of tumor tissue can reduce operation time and potentially improve patient outcomes in lung cancer operations. THG/SHG/2PEF imaging in seconds of fresh, unprocesessed human lung tissue is a promising tool for clinical intraoperative assessment of lung tumor tissue. In this study, this digital histology is compared with standard FFPE HE stained histology images.
Agreement between HHG and FFPE HE Slides
Fast and reliable intraoperative pathological feedback using Higher harmonic generation microscopy on unprocessed tissue within minutes, is a promising tool to avoid a two-stage procedure and significantly reduce health care costs in patients undergoing a diagnostic hemithyroidectomy. Images generated show relevant morphological thyroid structures in good accordance with the histology images.
Avoid two-staged thyroid cancer surgery
In lung surgery, immediate feedback on the nature of the excised tissue is important. A compact, transportable ex-vivo Third and Second harmonic generation (THG/SHG) microscope was tested for clinical use in this context. These are the first results of an ongoing study in which lung tumor tissue is imaged immediately after excision and frozen section biopsies, and compare the images to the golden standard histology.
Substantial agreement HHG and HE slides
